A8230
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 | Immunoblotting Cell lysates were separated on SDS-PAGE, blotted with Anti-Axin 1 (Cat. No. A8230), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. (Lane 1) HEK293 cells transfected with mouse FLAG-axin1 (10 μg cell lysate); (Lane 2) HEK293 cells transfected with mouse FLAG-axin1 (5 μg cell lysate); (Lane 3) HEK293 cells transfected with mouse FLAG-axin1 (10 μg cell lysate), antibody dilution 1:4,000 + Inhibition with AMPKa (human 2-19) immunizing peptide (20 μg/mL); (Lane 4) HEK293 cells (untransfected). |
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A8230
Sigma
Anti-Axin1 antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Axin, Anti-Axis inhibition protein 1, Anti-Axis Inhibitor-1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 604-624 located in the C-terminal region of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in mouse Axin1 and human has 76% identity. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~130 kDa |
| species reactivity | rat, mouse |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using HEK-293 cells expressing mouse FLAG-axin1 |
| Gene Information | rat ... Axin1(79257) |
| mouse ... Axin1(12005) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
